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Biotrend Chemicals cnqx
Cnqx, supplied by Biotrend Chemicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cnqx/cnqx/10__1523_slash_jneurosci__1956___25__2026-118-5-8
Average 86 stars, based on 1 article reviews
cnqx - by Bioz Stars, 2026-10
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Concentration Assay:

Article Title: All-trans retinoic acid induces synaptopodin-dependent metaplasticity in mouse dentate granule cells
Article Snippet: .. Reagent type (species) or resource Designation Source or reference Identifiers Additional information Chemical compound, drug All- trans retinoic acid (atRA) Sigma- Aldrich Cat#: R2625 Final concentration: 10 mg/kg Injection vehicle: Corn oil + 5% DMSO Chemical compound, drug Dimethyl Sulfoxide (DMSO) Sigma- Aldrich Cat#: D2650 Chemical compound, drug Paraformaldehyde (PFA) Carl Roth Cat#: 0335.3 Final concentration: 4% (w/v) in PB or PBS Chemical compound, drug Glutardialdehyd Carl Roth Cat#: 4157.2 Final concentration: 2% (w/v) in PB Chemical compound, drug CNQX Biotrend Cat#: BN0153 Final concentration: 10 μM Chemical compound, drug D- APV Abcam Cat#: ab120003 Final concentration: 10 μM Chemical compound, drug DAPI (1 mg/ml in water) Thermo Fisher Scientific Cat#: 62248 IF and post hoc labeling (1:5000) Lenz et al. eLife 2021;10:e71983. ..

Article Title: All-trans retinoic acid induces synaptopodin-dependent metaplasticity in mouse dentate granule cells
Article Snippet: Chemical compound, drug , Glutardialdehyd , Carl Roth , Cat#: 4157.2 , Final concentration: 2% (w/v) in PB. .. Chemical compound, drug , CNQX , Biotrend , Cat#: BN0153 , Final concentration: 10 μM. .. Chemical compound, drug , D-APV , Abcam , Cat#: ab120003 , Final concentration: 10 μM.

Injection:

Article Title: All-trans retinoic acid induces synaptopodin-dependent metaplasticity in mouse dentate granule cells
Article Snippet: .. Reagent type (species) or resource Designation Source or reference Identifiers Additional information Chemical compound, drug All- trans retinoic acid (atRA) Sigma- Aldrich Cat#: R2625 Final concentration: 10 mg/kg Injection vehicle: Corn oil + 5% DMSO Chemical compound, drug Dimethyl Sulfoxide (DMSO) Sigma- Aldrich Cat#: D2650 Chemical compound, drug Paraformaldehyde (PFA) Carl Roth Cat#: 0335.3 Final concentration: 4% (w/v) in PB or PBS Chemical compound, drug Glutardialdehyd Carl Roth Cat#: 4157.2 Final concentration: 2% (w/v) in PB Chemical compound, drug CNQX Biotrend Cat#: BN0153 Final concentration: 10 μM Chemical compound, drug D- APV Abcam Cat#: ab120003 Final concentration: 10 μM Chemical compound, drug DAPI (1 mg/ml in water) Thermo Fisher Scientific Cat#: 62248 IF and post hoc labeling (1:5000) Lenz et al. eLife 2021;10:e71983. ..

Article Title: All-trans retinoic acid induces synaptopodin-dependent metaplasticity in mouse dentate granule cells
Article Snippet: .. IV curves were generated by injecting 1 s square pulse currents starting at –100 pA and increasing in 10 pA steps until +500 pA injection was reached (sweep duration: 2 s). sIPSCs were recorded in the same extracellular solution by adding the AMPA receptor inhibitor CNQX (10 μM, Biotrend) and the NMDA receptor inhibitor APV (10 μM, Abcam). ..

Labeling:

Article Title: All-trans retinoic acid induces synaptopodin-dependent metaplasticity in mouse dentate granule cells
Article Snippet: .. Reagent type (species) or resource Designation Source or reference Identifiers Additional information Chemical compound, drug All- trans retinoic acid (atRA) Sigma- Aldrich Cat#: R2625 Final concentration: 10 mg/kg Injection vehicle: Corn oil + 5% DMSO Chemical compound, drug Dimethyl Sulfoxide (DMSO) Sigma- Aldrich Cat#: D2650 Chemical compound, drug Paraformaldehyde (PFA) Carl Roth Cat#: 0335.3 Final concentration: 4% (w/v) in PB or PBS Chemical compound, drug Glutardialdehyd Carl Roth Cat#: 4157.2 Final concentration: 2% (w/v) in PB Chemical compound, drug CNQX Biotrend Cat#: BN0153 Final concentration: 10 μM Chemical compound, drug D- APV Abcam Cat#: ab120003 Final concentration: 10 μM Chemical compound, drug DAPI (1 mg/ml in water) Thermo Fisher Scientific Cat#: 62248 IF and post hoc labeling (1:5000) Lenz et al. eLife 2021;10:e71983. ..

Blocking Assay:

Article Title: Optogenetic action potentials and intrinsic pacemaker interplay in retrogradely identified midbrain dopamine neurons.
Article Snippet: Subsequently, the slices were moved to a recording chamber and consistently perfused with ACSF at a rate of 2–4 ml/min, maintaining a temperature of 34 C using a Temperature Controller (Temperature Controller VI, Luigs Neumann, Germany). .. To block synaptic transmission CNQX (12.5 μM, Biotrend), DL-AP5 (10 μM, Tocris), and Gabazine (SR95531 4 μM, Biotrend) were added to the ACSF. .. Midbrain DA neurons were visualized through a light microscope (Axioskope 2 FS plus, Zeiss, Germany) coupled with an infrared light-source (SOLIS-850C, ThorLabs, USA) and a camera (CS505MUP1, ThorLabs, USA).

Transmission Assay:

Article Title: Optogenetic action potentials and intrinsic pacemaker interplay in retrogradely identified midbrain dopamine neurons.
Article Snippet: Subsequently, the slices were moved to a recording chamber and consistently perfused with ACSF at a rate of 2–4 ml/min, maintaining a temperature of 34 C using a Temperature Controller (Temperature Controller VI, Luigs Neumann, Germany). .. To block synaptic transmission CNQX (12.5 μM, Biotrend), DL-AP5 (10 μM, Tocris), and Gabazine (SR95531 4 μM, Biotrend) were added to the ACSF. .. Midbrain DA neurons were visualized through a light microscope (Axioskope 2 FS plus, Zeiss, Germany) coupled with an infrared light-source (SOLIS-850C, ThorLabs, USA) and a camera (CS505MUP1, ThorLabs, USA).

Article Title: KChIP4a is a biophysical amplifier of inhibition in atypical dopamine neurons and controls learning from negative prediction errors
Article Snippet: 268 After recovery, slices were transferred to a recording chamber and constantly perfused at a rate 269 of 2-4 mL/minute-1 with ASCF maintained at 37 °C (Temperature controller VI, Luigs Neumann, 270 Ratingen, Germany). .. Synaptic transmission was blocked using CNQX (12.5 μM, Biotrend), DL-271 AP5 (10 μM, Tocris) and Gabazine (4 μM, SR95531, Biotrend). .. Midbrain dopamine neurons were 272 visualized using a light microscope (Axioskope 2 FS plus, Zeiss, Oberkochen, Germany) and an 273 infrared camera (VX55, TILL photonics).

Article Title: Kv4.3 channel downregulation mediates chronic post-lesional pacemaker acceleration in surviving dopamine substantia nigra neurons
Article Snippet: .. CNQX (20 μM; Biotrend), gabazine (SR95531, 4 μM; Biotrend) and DL-AP5 (10 μM; Cayman Chemical) were added to inhibit excitatory and inhibitory synaptic transmission. .. Neurons were visualized using infrared differential interference contrast videomicroscopy with a digital camera (VX55, Till Photonics) connected to an upright microscope (Axioskop 2, FSplus, Zeiss).

Article Title: KChIP4a is a Biophysical Amplifier of Inhibition in Atypical Dopamine Neurons and Controls Learning from Negative Prediction Errors
Article Snippet: Slices were then transferred to a beaker containing ACSF (in mM: 125 NaCl, 3.5 KCl, 1.2 MgCl 2 , 1.2 CaCl 2 , 25 NaHCO 3 , 1.25 NaH 2 PO 4 constantly bubbled with carbogen) and allowed to recover at 37°C for 60 min. After recovery, slices were transferred to a recording chamber and constantly perfused at a rate of 2–4 ml/min −1 with ASCF maintained at 37°C (temperature controller VI, Luigs & Neumann). .. Synaptic transmission was blocked using CNQX (12.5 μM, Biotrend), d ʟ-AP5 (10 μM, Tocris), and gabazine (4 μM, SR95531, Biotrend). .. Midbrain dopamine neurons were visualized using a light microscope (Axioskop 2 FS plus, Zeiss) and an infrared camera (VX55, TILL Photonics).

Generated:

Article Title: All-trans retinoic acid induces synaptopodin-dependent metaplasticity in mouse dentate granule cells
Article Snippet: .. IV curves were generated by injecting 1 s square pulse currents starting at –100 pA and increasing in 10 pA steps until +500 pA injection was reached (sweep duration: 2 s). sIPSCs were recorded in the same extracellular solution by adding the AMPA receptor inhibitor CNQX (10 μM, Biotrend) and the NMDA receptor inhibitor APV (10 μM, Abcam). ..



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cnqx  (Tocris)
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Effects of serotonin and 5-HT receptor compounds on ARN KISS neuron firing in acute brain slices from diestrous female mice. (A) Robust excitatory effect of 90 seconds puff of 40 µM serotonin on firing rate of a middle ARN kisspeptin neuron. (B) Inhibitory effect of 90 seconds puff of 60 µM serotonin on firing rate of a caudal ARN kisspeptin neuron initially stimulated to fire by a 200 nM puff of NKB. (C) Rostral ARN kisspeptin neuron not responding to 60 µM puffs of serotonin but later activated by NKB. (D) Summary of percentage of rostral (rARN), middle (mARN), and caudal (cARN) kisspeptin neurons excited or inhibited by serotonin. (E) Caudal ARN kisspeptin neuron activated by serotonin (40 µM) in the absence and presence of methiothepin (100 µM). (F) Middle ARN kisspeptin neuron in which the excitatory effect of serotonin (40 µM) is blocked by methiothepin (100 µM). (G) Middle ARN kisspeptin neuron activated by serotonin (40 µM) in the absence and presence of SB228357 (100 µM). (H) Caudal ARN kisspeptin neuron in which zacopride (2 µM) facilitates serotonin excitation. (I) Whole-cell recording from an ARN KISS neuron in the continuous presence of TTX, <t>CNQX,</t> <t>DAP5,</t> and bicuculline, showing depolarization during bath application of 5-HT (30 µM). (J) Summary of mean membrane potential measured predrug, during 5-HT application, and following wash ( P < .001; n = 4 animals).
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MedChemExpress p re ss drugs nmda medchemexpress hy 17551 ampa medchemexpress hy 17551 cnqx medchemexpress hy
Effects of serotonin and 5-HT receptor compounds on ARN KISS neuron firing in acute brain slices from diestrous female mice. (A) Robust excitatory effect of 90 seconds puff of 40 µM serotonin on firing rate of a middle ARN kisspeptin neuron. (B) Inhibitory effect of 90 seconds puff of 60 µM serotonin on firing rate of a caudal ARN kisspeptin neuron initially stimulated to fire by a 200 nM puff of NKB. (C) Rostral ARN kisspeptin neuron not responding to 60 µM puffs of serotonin but later activated by NKB. (D) Summary of percentage of rostral (rARN), middle (mARN), and caudal (cARN) kisspeptin neurons excited or inhibited by serotonin. (E) Caudal ARN kisspeptin neuron activated by serotonin (40 µM) in the absence and presence of methiothepin (100 µM). (F) Middle ARN kisspeptin neuron in which the excitatory effect of serotonin (40 µM) is blocked by methiothepin (100 µM). (G) Middle ARN kisspeptin neuron activated by serotonin (40 µM) in the absence and presence of SB228357 (100 µM). (H) Caudal ARN kisspeptin neuron in which zacopride (2 µM) facilitates serotonin excitation. (I) Whole-cell recording from an ARN KISS neuron in the continuous presence of TTX, <t>CNQX,</t> <t>DAP5,</t> and bicuculline, showing depolarization during bath application of 5-HT (30 µM). (J) Summary of mean membrane potential measured predrug, during 5-HT application, and following wash ( P < .001; n = 4 animals).
P Re Ss Drugs Nmda Medchemexpress Hy 17551 Ampa Medchemexpress Hy 17551 Cnqx Medchemexpress Hy, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biotrend Chemicals cnqx
Effects of serotonin and 5-HT receptor compounds on ARN KISS neuron firing in acute brain slices from diestrous female mice. (A) Robust excitatory effect of 90 seconds puff of 40 µM serotonin on firing rate of a middle ARN kisspeptin neuron. (B) Inhibitory effect of 90 seconds puff of 60 µM serotonin on firing rate of a caudal ARN kisspeptin neuron initially stimulated to fire by a 200 nM puff of NKB. (C) Rostral ARN kisspeptin neuron not responding to 60 µM puffs of serotonin but later activated by NKB. (D) Summary of percentage of rostral (rARN), middle (mARN), and caudal (cARN) kisspeptin neurons excited or inhibited by serotonin. (E) Caudal ARN kisspeptin neuron activated by serotonin (40 µM) in the absence and presence of methiothepin (100 µM). (F) Middle ARN kisspeptin neuron in which the excitatory effect of serotonin (40 µM) is blocked by methiothepin (100 µM). (G) Middle ARN kisspeptin neuron activated by serotonin (40 µM) in the absence and presence of SB228357 (100 µM). (H) Caudal ARN kisspeptin neuron in which zacopride (2 µM) facilitates serotonin excitation. (I) Whole-cell recording from an ARN KISS neuron in the continuous presence of TTX, <t>CNQX,</t> <t>DAP5,</t> and bicuculline, showing depolarization during bath application of 5-HT (30 µM). (J) Summary of mean membrane potential measured predrug, during 5-HT application, and following wash ( P < .001; n = 4 animals).
Cnqx, supplied by Biotrend Chemicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cnqx/cnqx/10__1523_slash_jneurosci__1956___25__2026-118-5-8
Average 86 stars, based on 1 article reviews
cnqx - by Bioz Stars, 2026-10
86/100 stars
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Image Search Results


Effects of serotonin and 5-HT receptor compounds on ARN KISS neuron firing in acute brain slices from diestrous female mice. (A) Robust excitatory effect of 90 seconds puff of 40 µM serotonin on firing rate of a middle ARN kisspeptin neuron. (B) Inhibitory effect of 90 seconds puff of 60 µM serotonin on firing rate of a caudal ARN kisspeptin neuron initially stimulated to fire by a 200 nM puff of NKB. (C) Rostral ARN kisspeptin neuron not responding to 60 µM puffs of serotonin but later activated by NKB. (D) Summary of percentage of rostral (rARN), middle (mARN), and caudal (cARN) kisspeptin neurons excited or inhibited by serotonin. (E) Caudal ARN kisspeptin neuron activated by serotonin (40 µM) in the absence and presence of methiothepin (100 µM). (F) Middle ARN kisspeptin neuron in which the excitatory effect of serotonin (40 µM) is blocked by methiothepin (100 µM). (G) Middle ARN kisspeptin neuron activated by serotonin (40 µM) in the absence and presence of SB228357 (100 µM). (H) Caudal ARN kisspeptin neuron in which zacopride (2 µM) facilitates serotonin excitation. (I) Whole-cell recording from an ARN KISS neuron in the continuous presence of TTX, CNQX, DAP5, and bicuculline, showing depolarization during bath application of 5-HT (30 µM). (J) Summary of mean membrane potential measured predrug, during 5-HT application, and following wash ( P < .001; n = 4 animals).

Journal: Endocrinology

Article Title: Robust serotonin activation of the kisspeptin GnRH pulse generator in male and female mice

doi: 10.1210/endocr/bqag034

Figure Lengend Snippet: Effects of serotonin and 5-HT receptor compounds on ARN KISS neuron firing in acute brain slices from diestrous female mice. (A) Robust excitatory effect of 90 seconds puff of 40 µM serotonin on firing rate of a middle ARN kisspeptin neuron. (B) Inhibitory effect of 90 seconds puff of 60 µM serotonin on firing rate of a caudal ARN kisspeptin neuron initially stimulated to fire by a 200 nM puff of NKB. (C) Rostral ARN kisspeptin neuron not responding to 60 µM puffs of serotonin but later activated by NKB. (D) Summary of percentage of rostral (rARN), middle (mARN), and caudal (cARN) kisspeptin neurons excited or inhibited by serotonin. (E) Caudal ARN kisspeptin neuron activated by serotonin (40 µM) in the absence and presence of methiothepin (100 µM). (F) Middle ARN kisspeptin neuron in which the excitatory effect of serotonin (40 µM) is blocked by methiothepin (100 µM). (G) Middle ARN kisspeptin neuron activated by serotonin (40 µM) in the absence and presence of SB228357 (100 µM). (H) Caudal ARN kisspeptin neuron in which zacopride (2 µM) facilitates serotonin excitation. (I) Whole-cell recording from an ARN KISS neuron in the continuous presence of TTX, CNQX, DAP5, and bicuculline, showing depolarization during bath application of 5-HT (30 µM). (J) Summary of mean membrane potential measured predrug, during 5-HT application, and following wash ( P < .001; n = 4 animals).

Article Snippet: Stock solutions of GABAzine (SR95531, 5 mM, Tocris, UK), CNQX (10 mM, Tocris, UK), and DAP5, (50 mM, Tocris, UK) were prepared with Milli-Q water or NaOH for DAP5.

Techniques: Membrane